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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in lots of tumors, which ends up in chromosome instability, and so might lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital purpose in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two additional splice variants have been described but in-depth nucleotide sequence details isn't nonetheless offered.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide normally includes the epitope recognized with the antibody.
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This guidebook outlines additional possible optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This guideline outlines further doable optimization of mobile and immunoassay parameters to make certain the best possible results are...
Antibodies bound to the blocking peptide now not bind for the epitope within the concentrate on protein. By evaluating the staining in the blocked antibody as opposed to the antibody by itself, one can see which staining is particular.
Focus on Information Cyclin E belongs on the very conserved cyclin spouse and more info children, whose customers are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which add to your temporal coordination of each and every mitotic event. Cyclin E types a fancy with and functions as a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates on the G1-S phase boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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